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Linac Coherent Light Source (LCLS)

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  • Overview
  • Gas Dynamic Virtual Nozzles
  • Rayleigh Jets
  • High Viscosity Extruders
  • Droplet Injectors
  • Fixed Targets

High Viscosity Extruders

High Viscosity Extruders (HVE) are hydraulic micro-jetting devices designed to stream viscous, toothpaste-like materials.


How it works

High viscosity material is loaded into the reservoir and pushed by a plunger (polytetrafluoroethylene ball) through the short capillary (with 50-100 µm inner diameter). The polytetrafluoroethylene (PTFE) ball is driven here by a piston which additionally works as a hydraulic pressure amplifier. The ratio of cross-sections of the hydraulic stage bore and the sample reservoir bore will define the pressure amplification. The jet diameter in this case is approximately equal to the capillary inner diameter.

 

High Viscosity Injector
High Viscosity Injector (HVI). The hydraulic stage is pressurized by an HPLC pump. The piston pressurizes the sample in the reservoir and drives it through the capillary. The ceramic nozzle ensures that sheath gas surrounds the extruded high viscosity material and keeps the flow on the axis. (Weierstall et al.)

 

HVI devices usually use a sheath gas blowing through the specifically shaped gas aperture to keep the extruded jet stable and straight. The flow rate for HVI is typically 0.01-3 µL/min and jet speed 1-10 mm/s.

Types of high viscosity media

Different types of high viscosity media can be used to produce high viscosity jets. The most common media are Lipid Cubic Phase (LCP), poly-ethylene-oxide (PEO), Cellulose, and SuperLube. The choice of media depends on its stability as well as the stability of the crystal suspended in it. The sample consumption is significantly reduced for HVI compared to other liquid jet injection methods.

However, the significant jet thickness might reduce the noise-to-signal ratio. Additionally, high viscosity jets might produce strong diffraction rings which can mask low-resolution Bragg reflections.

 

A High Viscosity Extruder ejecting a gel stream through its nozzle

 

Pros and Cons of High Viscosity Extruders

ProsConsIdeal For
• Low sample consumption

• Low speed

• High background due to thick jet

• GPCR Crystals
LCLS | Biology Linac Coherent Light Source
2575 Sand Hill Road
Menlo Park, CA 94025
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